FIG. 6.
Interaction of karyopherins with in vitro-synthesized ORF29p. (Lanes 1, 3, and 4) ORF29p and (lanes 2 and 5) kap α2 were synthesized in a rabbit reticulocyte system in the presence of [35S]Met and mixed with either (lanes 4 and 5) GST alone or (lanes 1 to 3) GST-kap fusion proteins that were expressed in E. coli BL21 cells and bound to glutathione beads. (A) Bound proteins and (B) in vitro-translated protein inputs were subjected to SDS-PAGE analysis on a 4 to 12% gradient gel and exposed to film.