FIG. 1.

General protease inhibitors do not retard proteolytic processing of Hendra virus F. Vero cells transfected with pCAGGS-Hendra F were metabolically labeled and either lysed immediately (0 h) or chased for 2 h in DMEM in the absence (DMSO) or presence of a Complete protease tablet (without EDTA), 1 kU aprotinin, 1 mM PMSF, 100 μM leupeptin, 100 μM antipain, 100 μM chymostatin, 1 μM pepstatin A, 5 μM GM6001, or 0.1 mM 1,10-phenanthroline (o-Ph). Samples were analyzed by immunoprecipitation, 15% SDS-PAGE, and the Typhoon imaging system. Positions of uncleaved F₀ and cleaved F₁ and F₂ subunits are indicated.