FIG. 5.

RNA synthesis of the HCoV-229E Appr-1"-pase mutants HCoV_1ab-N1293A and HCoV_1ab-N1305. Shown is a Northern blot analysis of viral RNA synthesis at 72 h postinfection using poly(A) RNA from 6 × 10⁵ MRC-5 cells that had been infected with HCoV-229E (wild type [WT]), HCoV_1ab-N1293A, and HCoV_1ab-N1305A, respectively, at an MOI of 0.01 TCID₅₀ per cell. The ³²P-labeled probe used in this experiment was specific for the HCoV-229E 3′ terminal nucleotides 26857 to 27235.