FIG.6.
Northern blot analysis of transcripts of origin-flanking genes in Vero cells. Replicate monolayers of Vero cells (A and B) or rat embryonic cortical neurons (C and D) were infected with a calculated multiplicity of 10 PFU/cell with WT, DoriL-ILR, or DoriS-I. Infected cells were incubated at 37°C, and total cellular RNA was harvested at the indicated times. Total RNA (7.5 μg) was separated electrophoretically in 1% formaldehyde-agarose gels, blotted on nylon membranes, and hybridized to riboprobes specific for the indicated gene. Transcript levels were normalized to cellular 18S rRNA. Each experiment was standardized such that the maximum level of transcripts, plotted as arbitrary units, equaled 12. Representative Northern blots and the average levels (± SD) of transcripts from three to five experiments are shown graphically.