FIG. 2.

Replication compartment formation and cell size change. (A) Digital images of fluorescence micrographs of HEp-2 cells mock infected (i) or infected with wt HSV-1 (ii to vi). Cells were stained for ICP8 (red) and histone 1 (H1, green). Merged-channel images are shown to scale. (B) Box and whisker plot showing statistical analysis of nuclear cross-sectional areas at times following infection (2, 6, 8, 16, and 24 h) or mock infection (mock) with wt HSV-1. Cells were stained with antibodies to ICP8 and H1, and nuclear cross-sectional areas were measured using microscopy software (Zeiss Axiovision 5.2). Lines at top and bottom show data ranges, excluding outliers. Open boxes show first and third quartiles. Notches show 95% confidence intervals for medians, shown as the central horizontal line in each box. (C) Graph showing viral yields at points during a single cycle of viral replication. HEp-2 cells were infected with wt HSV-1, and total infectivity at different times was measured by plaque assay on Vero cells. Graph shows a representative example of replicate experiments.