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. 2005 Oct;79(20):13190–13194. doi: 10.1128/JVI.79.20.13190-13194.2005

FIG. 3.

FIG. 3.

Comparative infectivity of lentivirus in primary and established leukemia cells after concentration by either PMP or ultracentrifugation. Lentiviral vectors expressing enhanced green fluorescent protein (LV.gfp) were prepared from stable B7.1-expressing 293T cells (VSV-G) or 293T-Ampho (Ampho) and concentrated 100-fold either by CTLA4-Ig-conjugated PMP capture (PMP) or by ultracentrifugation (Ultra). After enzyme-linked immunosorbent assay determination of p24 Gag the viral concentrates were used at equimolar p24 levels to infect three cryopreserved primary AML samples (A to C; patients 1 to 3, respectively) and the established leukemia cell lines U937 and K562 (D and E, respectively) all in the presence of 4 μg/ml Polybrene. Fluorescence-activated cell sorting analysis of enhanced green fluorescent protein expression was carried out 96 h after infection (black line, enhanced green fluorescent protein; shaded area, background). No enhanced green fluorescent protein expression was detected for primary AML samples 2 and 3 following inoculation with amphotropic virus concentrated by either strategy (data not shown). ND, not detected.