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. 1983 Jan;47(1):57–63.

Effect of viral dose on experimental pneumonia caused by aerosol exposure of calves to bovine herpesvirus 1 and Pasteurella haemolytica.

W D Yates, K W Jericho, C E Doige
PMCID: PMC1235885  PMID: 6299485

Abstract

The effect of various aerosol doses of bovine herpesvirus 1, followed four days later by aerosol exposure to a constant level of Pasteurella haemolytica, was studied in 16 crossbred Hereford range calves. A Collision nebulizer was used to generate aerosols from virus suspensions with concentrations of 10(8.2) (high), 10(5.2) (moderate) or 10(2.2) (low) TCID50/mL. The bacterial suspension contained 10(7) colony forming units/mL. Control calves exposed only to P. haemolytica developed no pulmonary lesions. Calves in the low, moderate and high virus exposure groups developed lobular areas of atelectasis; in addition, one calf in the moderate and all four in the high virus exposure group developed fibrinous pneumonia. One of the latter calves died. The 50% effective dose for fibrinous pneumonia under these experimental conditions was 10(6.0) TCID50 bovine herpesvirus 1/mL of suspension in the nebulizer reservoir, and approximately 10(4.0) infectious units inhaled per calf.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brain J. D., Valberg P. A. Deposition of aerosol in the respiratory tract. Am Rev Respir Dis. 1979 Dec;120(6):1325–1373. doi: 10.1164/arrd.1979.120.6.1325. [DOI] [PubMed] [Google Scholar]
  2. Frank G. H., Marshall R. G. Relationship of serum and nasal secretion-neutralizing antibodies in protection of calves against parainfluenza-3 virus. Am J Vet Res. 1971 Nov;32(11):1707–1713. [PubMed] [Google Scholar]
  3. Gaskell R. M., Povey R. C. The dose response of cats to experimental infection with feline viral rhinotracheitis virus. J Comp Pathol. 1979 Apr;89(2):179–191. doi: 10.1016/0021-9975(79)90057-4. [DOI] [PubMed] [Google Scholar]
  4. Hales J. R., Findlay J. D. Respiration of the ox: normal values and the effects of exposure to hot environments. Respir Physiol. 1968 May;4(3):333–352. doi: 10.1016/0034-5687(68)90039-x. [DOI] [PubMed] [Google Scholar]
  5. Jakab G. J. Effect of sequential inoculations of Sendai virus and Pasteurella pneumotropica in mice. J Am Vet Med Assoc. 1974 Apr 1;164(7):723–728. [PubMed] [Google Scholar]
  6. Jakab G. J., Green G. M. Effects of pneumonia on intrapulmonary distribution of inhaled particles. Am Rev Respir Dis. 1973 Apr;107(4):675–678. doi: 10.1164/arrd.1973.107.4.675. [DOI] [PubMed] [Google Scholar]
  7. Jakab G. J. Mechanisms of virus-induced bacterial superinfections of the lung. Clin Chest Med. 1981 Jan;2(1):59–66. [PubMed] [Google Scholar]
  8. Jakab G. J. Suppression of pulmonary antibacterial activity following Sendai virus infection in mice: dependence on virus dose. Arch Virol. 1975;48(4):385–390. doi: 10.1007/BF01317437. [DOI] [PubMed] [Google Scholar]
  9. Jericho K. W., Darcel C. Q. Response of the respiratory tract of calves kept at controlled climatic conditions to bovine Herpesvirus 1 in aerosol. Can J Comp Med. 1978 Apr;42(2):156–167. [PMC free article] [PubMed] [Google Scholar]
  10. Jericho K. W., Langford E. V., Pantekoek J. Recovery of Pasteurella hemolytica from aerosols at differing temperature and humidity. Can J Comp Med. 1977 Apr;41(2):211–214. [PMC free article] [PubMed] [Google Scholar]
  11. Jericho K. W., Langford E. V. Pneumonia in calves produced with aerosols of bovine herpesvirus 1 and Pasteurella haemolytica. Can J Comp Med. 1978 Jul;42(3):269–277. [PMC free article] [PubMed] [Google Scholar]
  12. LAURENZI G. A., BERMAN L., FIRST M., KASS E. H. A QUANTITATIVE STUDY OF THE DEPOSITION AND CLEARANCE OF BACTERIA IN THE MURINE LUNG. J Clin Invest. 1964 Apr;43:759–768. doi: 10.1172/JCI104960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. May K. R. Multistage liquid impinger. Bacteriol Rev. 1966 Sep;30(3):559–570. doi: 10.1128/br.30.3.559-570.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith H. Mechanisms of virus pathogenicity. Bacteriol Rev. 1972 Sep;36(3):291–310. doi: 10.1128/br.36.3.291-310.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stockdale P. H., Jericho K. W., Yates W. D., Darcel C., Langford E. V. Experimental bovine pneumonic pasteurellosis. II. Genesis and prevention. Can J Comp Med. 1979 Jul;43(3):272–279. [PMC free article] [PubMed] [Google Scholar]
  16. Walker R. D., Corstvet R. E., Panciera R. J. Study of bovine pulmonary response to Pasteurella haemolytica: pulmonary macrophage response. Am J Vet Res. 1980 Jul;41(7):1008–1014. [PubMed] [Google Scholar]
  17. Yates W. D., Kozub G. C., Lejeune A. R. Comparison of Collison and De Vilbiss 65 nebulizers in the generation of aerosols for respiratory disease studies. Can J Comp Med. 1981 Oct;45(4):406–409. [PMC free article] [PubMed] [Google Scholar]

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