Skip to main content
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1985 Jan;49(1):43–49.

Chemotaxis of porcine neutrophils under agarose.

G S Smith, J H Lumsden, B P Wilcock
PMCID: PMC1236114  PMID: 3886107

Abstract

The standardization of pig neutrophil chemotaxis under agarose is described. The mean chemotactic index for pig neutrophils from six pigs measured over four days was 1.29. Comparative studies of human and pig neutrophil chemotaxis under agarose revealed a lower chemotactic index for pig neutrophils (mean of 1.18) compared to human neutrophils (mean of 2.43). The results suggest that this is due to differences intrinsic to human and pig neutrophils. In vitro pig neutrophil chemotaxis was measured in normal pigs and in pigs following experimental Salmonella typhimurium infection. Significant alterations in chemotaxis were not evident one and seven days postinfection.

Full text

PDF
43

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abramson J. S., Giebink G. S., Mills E. L., Quie P. G. Polymorphonuclear leukocyte dysfunction during influenza virus infection in chinchillas. J Infect Dis. 1981 Jun;143(6):836–845. doi: 10.1093/infdis/143.6.836. [DOI] [PubMed] [Google Scholar]
  2. Bowles C. A., Alsaker R. D., Wolfle T. L. Studies of the Pelger-Huët anomaly in foxhounds. Am J Pathol. 1979 Jul;96(1):237–248. [PMC free article] [PubMed] [Google Scholar]
  3. Camp C. J., Leid R. W. Chemotaxis of radiolabeled equine neutrophils. Am J Vet Res. 1982 Mar;43(3):397–401. [PubMed] [Google Scholar]
  4. Chenoweth D. E., Lane T. A., Rowe J. G., Hugli T. E. Quantitative comparisons of neutrophil chemotaxis in four animal species. Clin Immunol Immunopathol. 1980 Mar;15(3):525–535. doi: 10.1016/0090-1229(80)90064-1. [DOI] [PubMed] [Google Scholar]
  5. Clark R. A., Kimball H. R., Padgett G. A. Granulocyte chemotaxis in the Chediak-Higashi syndrome of mink. Blood. 1972 May;39(5):644–649. [PubMed] [Google Scholar]
  6. Cook L. O., Boxer L. A., Allen J. M., Griep J. A., Baehner R. L. In vitro functional capabilities of canine polymorphonuclear neutrophils collected simultaneously by continuous-flow centrifugation and continuous-flow filtration leukopheresis. Am J Hematol. 1978;4(3):225–232. doi: 10.1002/ajh.2830040304. [DOI] [PubMed] [Google Scholar]
  7. Frei P. C., Hermanovicz A., Pécoud A. Chemotaxis of human polymorphonuclears in vitro. V. Role of the nonsegmented neutrophils and of the experimental conditions in the impairment of chemotaxis observed during bacterial infections. J Lab Clin Med. 1978 Oct;92(4):577–584. [PubMed] [Google Scholar]
  8. Gallin J. I., Bujak J. S., Patten E., Wolff S. M. Granulocyte function in the Chediak-Higashi syndrome of mice. Blood. 1974 Feb;43(2):201–206. [PubMed] [Google Scholar]
  9. Hill H. R., Gerrard J. M., Hogan N. A., Quie P. G. Hyperactivity of neutrophil leukotactic responses during active bacterial infection. J Clin Invest. 1974 Apr;53(4):996–1002. doi: 10.1172/JCI107666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hill H. R., Warwick W. J., Dettloff J., Quie P. G. Neutrophil granulocyte function in patients with pulmonary infection. J Pediatr. 1974 Jan;84(1):55–58. doi: 10.1016/s0022-3476(74)80553-6. [DOI] [PubMed] [Google Scholar]
  11. Issekutz A. C., Biggar W. D. Influence of serum-derived chemotactic factors and bacterial products on human neutrophil chemotaxis. Infect Immun. 1977 Jan;15(1):212–220. doi: 10.1128/iai.15.1.212-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Issekutz A. C., Lee K. Y., Biggar W. D. Neutrophil chemotaxis in two patients with recurrent staphylococcal skin infections and hyperimmunoglobulin E. J Lab Clin Med. 1978 Oct;92(4):640–647. [PubMed] [Google Scholar]
  13. Jayappa H. G., Loken K. I. Enhancement of the chemotactic response of bovine polymorphonuclear leukocytes by levamisole. Am J Vet Res. 1982 Dec;43(12):2138–2142. [PubMed] [Google Scholar]
  14. Langloss J. M., Hoover E. A., Kahn D. E., Kniazeff A. J. Elaboration of chemotactic substances by alveolar cells: possible mechanisms for the initial neutrophilic response in feline caliciviral pneumonia. Am J Vet Res. 1979 Feb;40(2):186–189. [PubMed] [Google Scholar]
  15. Latimer K. S., Crane L. S., Prasse K. W. Quantitative evaluation of neutrophilic chemotaxis in beagles. Am J Vet Res. 1981 Jul;42(7):1254–1256. [PubMed] [Google Scholar]
  16. Latimer K. S., Prasse K. W., Dawe D. L. A transient deficit in neutrophilic chemotaxis in a dog with recurrent staphylococcal pyoderma. Vet Pathol. 1982 May;19(3):223–229. doi: 10.1177/030098588201900301. [DOI] [PubMed] [Google Scholar]
  17. Latimer K. S., Prasse K. W. Neutrophilic movement of a Basenji with Pelger-Huët anomaly. Am J Vet Res. 1982 Mar;43(3):525–527. [PubMed] [Google Scholar]
  18. McCall C. E., Caves J., Cooper R., DeChatlet L. Functional characteristics of human toxic neutrophils. J Infect Dis. 1971 Jul;124(1):68–75. doi: 10.1093/infdis/124.1.68. [DOI] [PubMed] [Google Scholar]
  19. Mowat A. G., Baum J. Polymorphonuclear leucocyte chemotaxis in patients with bacterial infections. Br Med J. 1971 Sep 11;3(5775):617–619. doi: 10.1136/bmj.3.5775.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Naidu T. G., Newbould F. H. A simple method for quantitation of chemotaxis of polymorphonuclear neutrophils in vitro. Immunol Commun. 1974;3(5):457–468. doi: 10.3109/08820137409061125. [DOI] [PubMed] [Google Scholar]
  21. Nelson R. D., Fiegel V. D., Simmons R. L. Chemotaxis of human polymorphonuclear neutrophils under agarose: morphologic changes associated with the chemotactic response. J Immunol. 1976 Nov;117(5 Pt 1):1676–1683. [PubMed] [Google Scholar]
  22. Nelson R. D., McCormack R. T., Fiegel V. D., Herron M., Simmons R. L., Quie P. G. Chemotactic deactivation of human neutrophils: possible relationship to stimulation of oxidative metabolism. Infect Immun. 1979 Feb;23(2):282–286. doi: 10.1128/iai.23.2.282-286.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
  24. Quie P. G., Cates K. L. Clinical conditions associated with defective polymorphonuclear leukocyte chemotaxis. Am J Pathol. 1977 Sep;88(3):711–726. [PMC free article] [PubMed] [Google Scholar]
  25. Repo H., Kosunen T. U. Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. II. Variables in the attraction assay. Scand J Immunol. 1977;6(3):211–218. doi: 10.1111/j.1365-3083.1977.tb00386.x. [DOI] [PubMed] [Google Scholar]
  26. Roth J. A., Kaeberle M. L. Effects of in vivo dexamethasone administration on in vitro bovine polymorphonuclear leukocyte function. Infect Immun. 1981 Aug;33(2):434–441. doi: 10.1128/iai.33.2.434-441.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Roth J. A., Kaeberle M. L. Evaluation of bovine polymorphonuclear leukocyte function. Vet Immunol Immunopathol. 1981 Apr;2(2):157–174. doi: 10.1016/0165-2427(81)90047-7. [DOI] [PubMed] [Google Scholar]
  28. Roth J. A., Kaeberle M. L., Griffith R. W. Effects of bovine viral diarrhea virus infection on bovine polymorphonuclear leukocyte function. Am J Vet Res. 1981 Feb;42(2):244–250. [PubMed] [Google Scholar]
  29. Roth J. A., Kaeberle M. L., Hsu W. H. Effects of ACTH administration on bovine polymorphonuclear leukocyte function and lymphocyte blastogenesis. Am J Vet Res. 1982 Mar;43(3):412–416. [PubMed] [Google Scholar]
  30. Roth J. A., Kaeberle M. L. Isolation of neutrophils and eosinophils from the peripheral blood of cattle and comparison of their functional activities. J Immunol Methods. 1981;45(2):153–164. doi: 10.1016/0022-1759(81)90209-x. [DOI] [PubMed] [Google Scholar]
  31. Shull R. M., Powell D. Acquired hyposegmentation of granulocytes (pseudo-Pelger-Huët anomaly) in a dog. Cornell Vet. 1979 Jul;69(3):241–247. [PubMed] [Google Scholar]
  32. Smith G. S., Lumsden J. H. Review of neutrophil adherence, chemotaxis, phagocytosis and killing. Vet Immunol Immunopathol. 1983 Mar;4(1-2):177–236. doi: 10.1016/0165-2427(83)90058-2. [DOI] [PubMed] [Google Scholar]
  33. Snyderman R., Pike M. C. N-Formylmethionyl peptide receptors on equine leukocytes initiate secretion but not chemotaxis. Science. 1980 Jul 25;209(4455):493–495. doi: 10.1126/science.6248959. [DOI] [PubMed] [Google Scholar]
  34. Zinkl J. G., Brown P. D. Chemotaxis of horse polymorphonuclear leukocytes to N-formyl-L-methionyl-L-leucyl-L-phenylalanine. Am J Vet Res. 1982 Apr;43(4):613–616. [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES