Figure 1.

Screening of anti‐prostate cancer agents in vitro with small‐molecule library. A,B) Prostate cancer cells in C4‐2B and 22Rv1 cells were plated in 96 well plates and indicated agents were added at the concentration of 10 µm and incubated for 48 h, the cell viability was determined with a CCK‐8 kit. Marked in the red star indicates the cell growth inhibition effect of the indicated agent is consistent in both C4‐2B and 22Rv1. n = 3. (C,D) The structures of six agents showed tumor cell growth inhibition effects in both C4‐2B and 22Rv1. E–G) The impact of 25 µm of Y8, A182, A193, B5, A94, and L14 on normal prostate RWPE1 cell (E) and the impact of 10 µm of ezetimibe on C4‐2B and 22Rv1 cell viability determined by the CCK‐8 kit. ^**, p < 0.01, ^* p < 0.05.