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. 2025 Jun 19;12(29):e04192. doi: 10.1002/advs.202504192

Figure 4.

Figure 4

L14‐8‐induced SAT1 transcriptional activation triggered ferroptosis. A) CCK‐8 assays were employed to determine the rescue effect of ferr‐1 on L14‐8 treatment‐induced cell growth inhibition in C4‐2B and 22Rv1 cells. B,C) RNA‐seq analysis of ferroptosis‐related gene expression analysis after L14‐8 treatment. D) Protein–protein interaction (PPI) analysis of DEGs with TP53. E) RT‐qPCR analysis of ferroptosis‐related gene expression analysis after L14‐8 treatment. F,G) The expression correlation of SAT1 with Glasson score (F) and patient survival (G) in MSK prostate cancer cohort. H,I) Gene ablation efficiency of different gRNAs corresponding to CRISPR‐Cas13 targeting SAT1. J,K) Cell viability was determined by CCK‐8 assays after SAT1 was knocked down and treated with L14‐8 in C4‐2B (H) and 22Rv1 (I) cells. **, p < 0.01.