Table 1.

Anisotropy of fluorescence observed in crystals of native GFP

Presence of polarizer	Presence of analyzer	Max-to-min fluorescence ratio
		Mean ± SD	n	Minimum, median, maximum
Yes	No	6.22  ± 1.00	16	4.73, 5.96, 8.11
No	Yes	5.93  ± 1.06	23	4.31, 5.78, 7.79
Yes	Yes*	29.61  ± 6.69	28	18.94, 29.57, 44.82
Yes	Yes†	1.23  ± 0.01	3	1.23, 1.23, 1.24‡

^*Polarizer transmission axis is parallel to analyzer transmission axis. 

^† Polarizer transmission axis is perpendicular to analyzer transmission axis. 

^‡ Ratio of minimum1-to-minimum2 [see supporting information III(iv) on the PNAS web site].