Figure 2.

Measurement of mitochondrial function in response to hyperosmotic conditions and glucose deprivation. (A) Mitochondrial activity in response to time of exposure to osmotic stress (600 mM sorbitol treatment) is compared with CCCP treatment. (B) Mitochondrial activity in response to level of osmotic stress (% sorbitol) is shown (black bars). Kit-225 cells were treated with sorbitol for 30 min before measuring mitochondrial activity. Mitochondrial activity 20 min after removal of osmotic stress (withdrawal of sorbitol) is shown as adjoining bars for each dose (gray bars). (C) Mitochondrial activity in response to glucose deprivation. Kit-225 cells were exposed to conditions indicated and mitochondrial proton gradient was measured by JC-1 assay. CM, complete media; GDM, glucose-deprived media.