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. 2025 Aug 4;11(8):1670–1680. doi: 10.1038/s41477-025-02056-z

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a. Workflow of image processing and 3D reconstruction of apo LAX3. The processing was done in CryoSPARC v. 4.4.0. b. Representative micrograph showing the particle distribution of 10273 micrographs. c. Sharpened density map from the final non-uniform refinement colored by local resolution. d. Heat map showing the orientation distribution of the final particle stack. e. The corrected curve of the global Fourier shell correlation (FSC) indicates 3.16 Å based on the 0.143 gold-standard criterion. f. Representative size-exclusion chromatography profile and corresponding SDS-PAGE analysis of a LAX3 purification. Four individual purifications were carried out, one for each cryo-EM structure. g. Dimeric LAX3 2D-classes from a small subset of the particles.