Extended Data Fig. 3. Deviant flg22s induce ROS but not MAPK phosphorylation.
a, Normalized ROS induction of flg22 variants (100 nM concentration) in N. benthamiana fls2-1/2 expressing QvFLS2. Each data point represents an average max relative light unit (RLU) from 4 plants with 4 leaf disks per plant. The values of each plate were normalized by adjusting maximum relative light unit (RLU) averages to a 0 (water) to 100000 (Pae) scale. A one-way ANOVA and Dunnett’s multiple comparison were used to determine significance using the non-scaled data, p values from left to right are marked on top. Error bars = SD. b, ROS curves for two different concentrations (100 nM and 1 μM) of Atu and Pvi. Data are presented as mean values ± SEM, n = 4 leaf disks. c, Normalized phosphorylated MAPK abundance. Bands from all treatments at 15 mins were normalized to Pae 15 mins on the same blot, then analyzed collectively. A one-way ANOVA and Dunnett’s multiple comparison were used to determine significance using the non-scaled data, p values from left to right are marked on top. Data are presented as mean values ± SD. Data points were derived from 10 independent blots, with each treatment repeated at least three times. d, Phosphorylation of MAPK induced by flg22 variants in the N. benthamiana fls2-1/2 expressing QvFLS2. CBB = protein loading. All experiments were repeated at least three times with similar results.