(A) Predicted amino acid sequence of TbPDE2C.
Boxed sequences represent GAF domains A and B. The central core of the
catalytic domain is underlined, and the PDE signature sequence is
marked by black dots. (B) Amino acid sequence identity
between the catalytic cores of class I PDE families. 1, human PDE 1C3A
(accession no. U40372); 2, human PDE2A (NP002590); 3, human PDE 3A
(M91667); 4, human PDE4A4B (AAC35012); 5, human PDE5A (NM_001083); 6,
human PDE6B (NM_000283); 7, mouse PDE7A2 (U68171); 8, mouse PDE8
(NM_008803); 9, mouse PDE 9A (AF031147); 10, human PDE 10A
(AF127479); 11, human PDE 11A1 (AJ251509); 12, T. brucei
TbPDE2A (AF263280); 13, T. brucei TbPDE1 (AF253418); 14,
D. discoideum regA (U60170); 15, D.
melanogaster dunce (P12252); 16, S. cerevisiae
pde2 (M14563). (C) Complementation of the heat-shock
phenotype of S. cerevisiae PP5-12 by TbPDE2C. Row 1,
First and last patches: PP5-12 containing the empty vector pLT1
(negative control); second and third patches: PP5-12 expressing TbPDE1
(positive control). Row 2, Two patches of PP5-12 expressing untagged
TbPDE2C. Row 3, Two patches of PP5-12 expressing TbPDE2C with a
C-terminal hemagglutinin tag. −, No heat shock; +, with heat shock.