Figure 4.
(A) Increase in intracellular cAMP in procyclic trypanosomes after RNAi-mediated inactivation of the TbPDE2 family. C, procyclic strain 29-13 (control). RNAi directed against: 1, TbPDE1; 2, the entire TbPDE2 family; 3, TbPDE2A; 4, TbPDE2B; 5, TbPDE2C. −, No induction of RNAi; +, RNAi induced by 1 μg/ml tetracycline. Gray bars, 48 h after induction; black bars, 120 h after induction. Intracellular cAMP level in control cells was 64 ± 11 pmol/109 trypanosomes. (B) Susceptibility of the proliferation of bloodstream form trypanosomes to 8-bromo-cAMP.