Table 1.
cup-5L, cup-5S, and hML-IV rescue cup-5(n3194) lethality
| Transgene | Heat shock | Total no. eggs laid | Total no. eggs hatched | Eggs hatched, % | No. of adults |
|---|---|---|---|---|---|
| Phsp alone | − | 178 | 0 | 0 | 0 |
| Phsp alone | + | 360 | 3 | 0.8 | 1 |
| Phspcup-5L | − | 232 | 28 | 12.1 | 24 |
| Phspcup-5L | + | 301 | 127 | 42.2 | 118 |
| Phspcup-5S | − | 178 | 26 | 14.6 | 24 |
| Phspcup-5S | + | 271 | 135 | 49.8 | 121 |
| PhsphML-IV | − | 136 | 0 | 0 | 0 |
| PhsphML-IV | + | 263 | 24 | 9.1 | 21 |
| PhsphML-IV(fs) | − | 243 | 0 | 0 | 0 |
| PhsphML-IV(fs) | + | 314 | 1 | 0.3 | 0 |
Transgenic extrachromosomal arrays carried by unc-36(e251) cup-5(n3194)/qC1 animals were tested for rescue as described in Materials and Methods. Animals were removed from Petri plates 24 h following heat-shock treatment, and the number of eggs present on each plate was counted. Hatching was assayed 2 days after heat-shock treatment, and the presence of adult animals was assayed 4 days after heat-shock treatment. At least three transgenic lines were tested for each construct. Data for a single representative line are shown here. fs, frameshift.