Table 3.
Transgene | Heat-shock | Total no. eggs laid | Total no. hatched | Hatched, % |
---|---|---|---|---|
Phsp p35 | − | 253 | 2 | 0.8 |
Phsp p35 | + | 351 | 27 | 7.7 |
Phsp p35 | + 2x | 187 | 16 | 8.6 |
At least 10 cup-5(n3194) unc-36(e251) animals carrying a transgenic array of p35 were subjected to a single pulse of 33°C heat-shock (+) or two pulses of heat-shock separated by 1 hr recovery at 20°C (+ 2x). The total numbers of eggs laid and hatched were counted.