Fig. 3.

Phenotypic comparisons between CD18^-/- mice and their WT sex-matched littermates. (A) Faxitron analysis demonstrated a decreased bone density in the femurs of 5-week-old CD18^-/- mice (KO, Right) as compared with their WT counterparts (Left). (B) Dual x-ray absorptiometry analysis of the femurs from 15-week-old mice showed a statistically significant difference in BMD between WT (left bar) and CD18^-/- (right bar) mice (P = 0.025, n = 4). (C) Hematoxylin/eosin staining on the metaphysis area of the femurs showed diminished trabecular bone structure in CD18^-/- (Right) mice as compared with WT mice (Left). (Magnification: ×400.) (D) Representative images of the distal femur metaphysis on microcomputed tomography analysis revealed decreased bone volume, trabecular bone number, trabecular bone thickness, and an increased trabecular bone space. (E) Alizarin red and Alcian blue double skeletal staining for bone (red) and cartilage (blue) of 1-week-old mice. KO, CD18^-/-