Table 1. CD, sedimentation equilibrium AUC, and SLS data for ccCor1 WT and variants.
Peptides | [Θ]222* (103 deg·cm2·dmol-1) | Tm,† °C | MWobs/MWmonomer,‡ 50 μM/100 μM/200 μM | SLS,§ kDa |
---|---|---|---|---|
ccCor1 | -28.4 | 79 | 3.1/3.1/3.0 | 10.9 (3.8) |
ccCor1-R450A | -32.2 | 67 | 3.8/3.8/4.0 | 13.8 (3.7) |
ccCor1-R450Nle | -24.7 | 86 | 4.0/3.9/4.1 | 14.8 (3.8) |
ccCor1-R450K | -23.0 | 66 | 3.1/3.3/3.8 | n.d. |
[Θ]222 measured at 5 °C at peptide concentrations (monomer) of 35 μM.
Tm determined at peptide concentrations (monomer) of 35 μM.
Average molecular masses determined by AUC.
Average molecular masses determined by SLS. The molecular masses of the monomers are given in parentheses. n.d., not determined.