Fig. 2.

SKS1, IMP3, and YLR194C mRNAs are stabilized by deletion of Cbc1p and Rrp6p. Normal, cbc1-Δ, and rrp6-Δ cells were grown at 30°C to an A₆₀₀ = 1.0 and then treated with 4 μg/ml thiolutin, and samples were removed from the culture at various times after the treatment as indicated at the top of each panel. Total RNAs were extracted from each sample, electrophoresed, and blotted, and the decay of the specific mRNA was determined by Northern blot analysis. The blot was sequentially hybridized with random priming probe specific to the SKS1, IMP3, YLR194C, ACT1, and CYH2 as noted. The half-life in min of each mRNA is shown to the right of the panel. The signal values were normalized to SCR1 RNA.