Fig. 6.

Effects of phase 2 inducers and COX-2 inhibitors on shear-mediated DNA fragmentation, mitochondrial membrane permeabilization, and caspase-9 protein levels. T/C-28a2 cells were treated with the solvent (0.1% DMSO) or D3T (5 μM), for 24 h or solvent CAY10404 (6.75 μM) or NS398 (30 μM) for 2 h, and then exposed to either static or laminar flow (20 dyn/cm²) for 48 h in the presence of the agent. Cells were examined for markers of apoptosis by using DNA fragmentation (TUNEL, A), mitochondrial membrane potential (MMP, B), and caspase-9 expression (C)