Figure 6.

Transplantation of embryonic endothelial cells (PECAM1+) in SCID mice. PECAM1+ cells were seeded onto PLLA/PLGA polymer scaffolds as described in Materials and Methods. The cells plus scaffolds were implanted s.c. in the dorsal region of 4-week-old SCID mice. (A–C) Immunoperoxidase (brown) staining of 7-day implants with anti-human PECAM1 antibodies and (D and E) of 14-day implants with anti-human CD34 antibodies, showing microvessels that are immunoreactive with these human-specific antibodies. Some of these human-positive microvessels have mouse blood cells in their lumen (magnification, ×400).