Table 1. Nomenclature of strains and proteins of A. vinelandii related to this work.
Strain | Strain designation | Source | Genotype | Designation of component 1* | Designation of component 2* |
---|---|---|---|---|---|
Mo-nitrogenase | |||||
WT | 26 | WT | Av1 | Av2 | |
DJ1143 | AvDJ1143 | 21 | nifB deletion, His-tagged MoFe protein | ΔnifB Av1 | |
DJ1165 | AvDJ1165 | 17 | nifH deletion, His-tagged MoFe protein | ΔnifH Av1 | |
DJ1165 | AvDJ1165 | This study | nifH deletion, His-tagged MoFe protein, preincubated with Av2 and MgATP | ΔnifHAv1′ | |
V-nitrogenase | |||||
WT | 27-29 | WT | Av1v | Av2v | |
YM7A | AvYM7A | This study | nifB deletion, His-tagged VFe protein | ΔnifB Av1v |
The component proteins of the Mo-nitrogenase are designated by the initials of the organism from which they are isolated and the Arabic numeral of the component. For example, the MoFe and Fe proteins of the Mo-nitrogenase of A. vinelandii are designated Av1 and Av2, respectively. This nomenclature is also applied to the V-nitrogenase, with the addition of a superscript V. Therefore, the VFe and Fe proteins of the V-nitrogenase of A. vinelandii are abbreviated as Av1v and Av2v, respectively.