FIG. 3.
Western immunoblot analysis of P30 and P65 in wild-type, mutant, revertant, and transformant M. pneumoniae. (A and B) Western immunoblot analysis of the indicated strains probed with P30-specific antibodies. II-3 + P30, mutant II-3 transformed with the indicated recombinant P30 allele by transposon delivery. (B) Samples processed to allow resolution of the multimeric forms of P30 from II-3R (arrows). (C) Western immunoblot analysis of parallel samples from panel A by use of P65-specific antibodies (top panel) or P1-specific antibodies as an internal control (bottom panel).