FIG. 5.

Scc2 and Scc3 coprecipitate CopB from C. trachomatis-containing lysates. Scc1-FT, Scc2-FT, Scc3-FT, and PBS as a mock-treated control (Mock) were incubated with cleared lysates from purified C. trachomatis RB and EB developmental forms. Flag-tagged proteins were purified by addition of α-FlagM2 resin, and material was analyzed by immunoblotting with CopB-, CopB2-, Hsp60-, or FT-specific antibodies. Lanes containing whole-culture lysates from mock-infected (HeLa) or C. trachomatis-infected (HeLa + L2) HeLa cell cultures were included as a control for antibody specificity. Proteins were visualized by probing with alkaline phosphatase-conjugated secondary antibodies, followed by development with NBT-BCIP.