TABLE 3.
ATP retention and LDH release
| Treatment | Alpha-toxin (μg/ml) | Biotin (μg/ml) | [ATP] (relative units [± SD])a | % Controlb | LDH activity (U/ml [± SD])c | % Controlb |
|---|---|---|---|---|---|---|
| Mock | 0.00 | 750.0 | 82.2 (± 1.22) | 100 | 615.5 (± 133) | 5.92 |
| Mock | 50.0 | 750.0 | 15.1 (± 1.08) | 18.4 | 1912 (± 6.36) | 18.4 |
| Mock | 50.0 | 0.000 | 11.4 (± 2.42) | 13.9 | 1867 (± 43.3) | 17.9 |
| L2 | 0.00 | 750.0 | 117 (± 7.52) | 100 | 1147 (± 135) | 11.0 |
| L2 | 50.0 | 750.0 | 15.2 (± 2.01) | 12.9 | 1904 (± 20.5) | 18.3 |
| L2 | 50.0 | 0.000 | 13.3 (± 1.22) | 11.4 | 1901 (± 13.4) | 18.3 |
a
Values correspond to quantitation of ATP-dependent bioluminescence in sample dilutions falling within the linear range of a standard curve and reflect levels of ATP retained by respective cultures.
b
Percent control represents activity in a fractionated culture sample compared to activity in an unfractionated, parallel culture.
c
Values represent LDH activity released by HeLa monolayers into the culture medium.