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. 2025 Jul 24;91(8):e01136-25. doi: 10.1128/aem.01136-25

Fig 3.

SDS-PAGE confirms NmCut expression. Activity assays indicate optimal temperature at 60 °C and pH 8.0. The enzyme shows increased activity in the presence of K⁺, Na⁺, Ba²⁺, Mg²⁺, and Al³⁺ ions. It remains 68% active after 48 hours of incubation at 60 °C.

Catalytic properties of NmCut. (A) SDS-PAGE gel showing the protein purity of NmCut. (B) Optimal reaction temperature of NmCut. (C) Optimal reaction pH of NmCut. (D) Effect of metal ions on the activity of NmCut. (E) Thermal stability of NmCut at 60°C. All assays were conducted in triplicate using p-nitrophenyl butyrate (pNP-butyrate) as the substrate. Data are shown as mean ± SD (n = 3).