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. 2025 Jul 24;91(8):e01136-25. doi: 10.1128/aem.01136-25

Fig 4.

SEM images reveal surface degradation of PET by NmCut after 12 and 24 h, while blank remains unchanged. Bar graph shows increasing product concentrations over time, with NmCut_PETP yielding the highest activity, followed by NmCut_PETF and NmCut1_PETP.

PET degradation activity of NmCut. PET (15 mg) was incubated with 1 µM NmCut in pH 8.0 buffer for 24 h at 60°C. (A) SEM images showing the surface morphology of PET degradation by NmCut at 60°C. (B) Quantification of degradation products (TPA, MHET, BHET) released by NmCut and NmCut1 (NmCut without helix) in pH 8.0 potassium phosphate buffer over 48 h at 60°C. PETP is 11.3% crystallinity PET powder, and PETF is 7.1% crystallinity PET film. Data are shown as mean ± SD (n = 3).