TABLE 2.

Reactivity of L. paracasei Shirota (YIT 9029) and mutants to L. paracasei YIT 9029-specific monoclonal antibody (21)^a

Antibody reactivity	Strain
Positive	YIT 9029 (wild type), Ω0209, Ω0211, Ω0214, Ω0215, Ω0216, Ω0228, Ω0229, Ω0230, Ω0231, Ω0661, Ω0704, Ω0705, Ω0822, Ω0884, Ω0885, Ω1062, Ω1063, Ω1064, Ω1065, Ω1111, Ω1128, Ω1889, Ω1892, Ω1893 (rmlD1), Ω1894 (rmlB1), Ω1895 (rmlC1), Ω1896 (rmlA1), Ω1898, Ω1899, Ω1926, Δ1932 (rmlD2), Ω1934 (rmlC2), Ω1935 (rmlA2), Ω1937 (cps1I), Ω1938 (cps1H), Ω2708, Δcps1C/cps1C, YIT 9022
Slightly positive	Ω0212, Ω0213, Ω0823, Ω0824, Ω0838, Ω1927, Ω1933 (rmlB2), Ω1940 (cps1F), YIT 9021
Negative	Ω1945 (cps1A), Ω1944 (cps1B), Δ1943 (cps1C), Ω1942 (cps1D), Ω1941 (cps1E), Ω1939 (cps1G), Ω1936 (cps1J), YIT 9036, YIT 9037, YIT 0180

^a The reactivity of the mutants to YIT 9029-specific monoclonal antibody was determined using a sandwich enzyme-linked immunosorbent assay, as described previously (20). The resultant fluorescence intensities of wild type and mutants were classified into three types: “positive” with full to half of the color intensity as that of wild-type YIT 9029, “negative” with very weak or no color, and “slightly positive” with weak or slight color intensity. An absorbance of ≥1.0 was considered positive and <0.25 was considered negative. The absorbance of ≥0.25 but <1.0 was considered slightly positive.