TABLE 2.

PCR analysis of plasmid DNA isolated from CS1-positive ETEC strains^a

Class and strain	Serotype	CS pili	Toxinsb		Amplified regionsc												Tcf
			LT	ST	pCoo specific	R64 pilus			R64 ori	Coo	DRI		R100 ori	EatA	DRII
					A	B	C	D	E	F	G	H	I	J	K	L
Class A
C921b-1	O6H16	1, 3	+	+	+	+	+	+	+	+	+	+	+	+	+	+	S
1392-75-2A	O6H16	1, 3	−	?	+	+	+	ND	+	+	+	+	+	+	+	+	S
M424CI	O6H16	1, 3	?	?	+	+	+	ND	+	+	+	+	+	+	+	+	S
TW03605	O6H16	1, 2, 3, 4	+	+	+	ND	+	+	+	+	+	+	+	+	+	+	S
TW03923	O6H−	1, 2, 3, 6	+	+	+	ND	−	−	+	+	+	+	+	+	+	+	S
TW03875	O6	1, 3	+	+	+	ND	+	+	+	+	+	+	+	+	+	+	S
TW04215	O6	1, 3	+	+	+	ND	+	ND	+	+	+	+	+	+	+	+	S
TW04205	O6	1, 2, 6	+	+	+	ND	+	+	+	+	+	+	+	+	+	+	S
60R70	O6	1	+	?	+	−	−	+	+	+	+	+	+	+	+	+	S
294		1	?	?	+	+	+	ND	+	+	+	+	+	+	+	+	S
295		1	?	?	+	+	+	ND	+	+	+	+	+	+	+	+	S
296		1	?	?	+	−	+	ND	+	+	+	+	+	+	+	+	R
Class B
TW04244	O−	1	+	+	+	ND	−	+	+	+	−	+	+	+	−	−	R
TW04144	O−	1, 2, 4, 5, 6	−	+	+	ND	+	+	+	+	−	+	+	+	+	+	S
TW03677	O25	1	+	−	+	ND	−	−	+	+	+d	+	+	−	−	−	S
TW04237	O27	1, 3, 4, 6	−	+	+	ND	+	+	+	+	+	+	+	+	−	+	S
E24377/O/A	O139H8	1, 3	+	+	+	ND	−	+	+	+	−	+	+	+	−	−	S
TW04316	O148	1, 2, 6	−	+	+	ND	−	+	+	+	−	+	+	+	−	−	S
TW04211	O159	1, 6	−	+	+	ND	−	+	+	+	+	+e	+	+	+	+	S

^aThe table is divided into two parts, classes A and B by O type and the presence of DRI and DRII PCR products identical to those for C921b-1.

^b?, unknown.

^cThe amplified fragments G, H, K, and L each use one primer within the direct repeat and the second flanking the repeat. ND, not done.

^dPCR product larger than that for C921b-1.

^ePCR product smaller than that for C921b-1.

^fS, sensitive; R, resistant.