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. 2005 Sep;187(18):6430–6442. doi: 10.1128/JB.187.18.6430-6442.2005

FIG. 5.

FIG. 5.

UV induction and promoter activities of RP mutants. (A) UV induction of single lysogens. All phages carried cI D38R and the indicated promoter allele, except for the wild type (WT). (B) UV induction of single lysogens. Except for the wild type, all phages carried cI D38N and the indicated promoter allele. (C) Promoter activity of D38R suppressors in the presence of CI D38R. Single lysogens of phages bearing PRM::lacZ protein fusions were prepared and assayed as described in legend to Fig. 4, except that the second strain carried pJWL710, which expresses CI D38R. In the wild-type control, wild-type CI was provided from plasmid pA3B2. All phages carried a variant of OL located distal to the lacZ reporter. For each curve, the PRM allele in the reporter fusion is given, followed by the allele of cI carried on the plasmid. (D and E) Promoter activities of D38R suppressors in the presence of the wild type (D) or CI D38N (E). The experiment was as in panel C, except that cells with the CI-bearing plasmid carried pJWL709 or pA3B2, which expresses CI D38N or wild-type CI, respectively. Curves are labeled as for panel C.