TABLE 2.
Sequences and properties of PRM variants isolated with cI D38Na
| PRM allele | −35 region | −10 region | cI allele or no. of isolates | Set point | Maximum level of β-galactosidase with cI D38N (except as noted)
|
|
|---|---|---|---|---|---|---|
| −OL | +OL | |||||
| WT | TAGA | GATT | cI+ | 12 | 266, 280 (cI+) | 190, 168 (cI+) |
| WT | TAGA | GATT | cI D38N | ∼0.8 | 54 (cI D38N) | 34 (cI D38N) |
| Mutants containing a PRM variant and cI D38N | ||||||
| NP1b | GGAA | AGAT | 4 | 15 | NA | NA |
| NP2 | GCTG | TATT | 1 | 7 | 140 | 85 |
| NP3 | CATT | GAAT | 2 | 4 | ND | ND |
| NP4 | CCTT | CCAT | 1 | 3.5 | 102 | 73 |
| NP5 | CTAA | GAAT | 2 | 8 | 171 | 114 |
| NP6 | CCCA | TGAT | 1 | 5 | ND | 120 (not shown) |
| NP7 | TACC | TACT | 1 | 4 | ND | ND |
| NP8 | GTGT | GTAT | 1 | 15 | ND | 217 (not shown) |
| NP9 | GCAC | GGAT | 14 | 8 | 179 | 117 |
| NP10 | CCAA | GAAT | 1 | 14 | 246 | 174 |
| NP11 | CTCA | TGCT | 1 | 3 | ND | ND |
| NP12 | CTGA | TAAT | 1 | 8 | ND | 78 (not shown) |
a
Only positions 3 to 6 for each of the −35 and −10 regions are shown. Positions 1 and 2 are TA in both cases. The set point is the dose of UV giving 50% of the maximal phage yield. ND, not done; NA, not applicable.
b
The mutant carrying PRM-NP1 also has a mutation of OR2 (CAACGCGCACGGTGT; the underlined base is A in the wild type).