Figure 3.

Three-dimensionally reconstructed CTs in great apes and white-handed gibbon nuclei. (a) Three-dimensional positioning of HSA18 (red) and HSA19 (green) CTs in a human lymphoblastoid cell nucleus with the partially reconstructed nuclear border (outside, blue; inside, silver-gray). (b–d) Three-dimensional positioning of HSA18 and HSA19-homologous CTs in nuclei of great apes. In correspondence with the situation in human lymphoblastoid cell nuclei, the HSA18-homologous CTs were always positioned close to the nuclear border. The relative locations of the CTs, however, varied from a close neighborhood to opposite positions (for example, compare b and c). (e and f) Two gibbon nuclei exemplify the variability but still consistently interior location of reshuffled HSA19-homologous chromosome segments in this species of lesser apes in contrast to the peripherally located HSA18-homologous segments. The origin of paint probes used for each species is described in Table 1.