TABLE 1.
Bacterial strains, plasmids, and phages used in this study
| Strain, plasmid, or phage | Description, relevant properties, or use | Reference and/or source |
|---|---|---|
| E. coli | ||
| DH5α F′lacIQ | General-use host strain for cloning plasmids | Invitrogen |
| AG1688 | MC1061 F′128 lacIqlacZ::Tn5 | J. Hu (15) |
| JH372 | AG1688(λ202) = λPROR(OR2−)-lacZ reporter strain | J. Hu (15) |
| JH607 | AG1688(λ112OsPs) = λOsPs-lacZ reporter strain | J. Hu (15) |
| XZ980 | AG1688(λXZ970) = λOSPS(OR1434)-lacZ reporter | J. Hu (15) |
| TP611 | pcn mutant for low-copy-number cloning | K. Devine |
| B. subtilis | ||
| SWV994 | trpC2 spo0A12 tolB24 ΔthrC::abrB8-lacZ spcr | Lab strain |
| SWV1030 | SWV994 ΔamyE::pSpac-abrB, cmr | Lab strain |
| FYS75 | SWV994 ΔamyE::(pFY80) | This study |
| FYS76 | SWV994 ΔamyE::(pFY81) | This study |
| Plasmids | ||
| pJH391 | Cloning vector for cI DBD fusion constructs | J. Hu (15) |
| pFG157 | pJH391 with intact λcI | J. Hu (15) |
| pKH101 | pJH391 with cI DBD only | J. Hu (15) |
| pJH370 | pJH391 with cI-GCN4 (dimeric) | J. Hu (15) |
| pJH622 | pJH391 with cI-GCN4 (tetrameric) | J. Hu (15) |
| pFY7 | cI DBD fused to AbrB 48-94 | This study |
| pFY8 | cI DBD fused to SpoVT 50-178 | This study |
| pFY12 | cI DBD fused to AbrB 48-80 | This study |
| pFY13 | cI DBD fused to AbrB 48-94 L67P | This study |
| pFY15 | cI DBD fused to Abh 50-92 | This study |
| pFY16 | cI DBD fused to AbrB 27-94 | This study |
| pFY17 | cI DBD fused to SpoVT 92-178 | This study |
| pFY18 | cI DBD fused to AbrB 67-94 | This study |
| pFY20 | cI DBD fused to SpoVT 50-131 | This study |
| pFY21 | cI DBD fused to SpoVT 92-131 | This study |
| pFY22 | cI DBD fused to SpoVT 124-178 | This study |
| pFY28 | cI DBD fused to AbrB 4-53 | This study |
| pFY31 | cI DBD fused to AbrB 55-94 | This study |
| pFY32 | cI DBD fused to AbrB 4-94 | This study |
| pFY34 | cI DBD fused to SpoVT 59-178 | This study |
| pFY36 | cI DBD fused to SpoVT 59-131 | This study |
| pFY37 | cI DBD fused to SpoVT 4-160 | This study |
| pFY38 | cI DBD fused to SpoVT 59-160 | This study |
| pFY39 | cI DBD fused to Abh 55-92 | This study |
| pFY46 | cI DBD fused to AbrB 4-53 R24S | This study |
| pFY47 | cI DBD fused to AbrB 4-53 L34H | This study |
| pFY48 | cI DBD fused to AbrB 51-94 | This study |
| pFY49 | cI DBD fused to AbrB 53-94 | This study |
| pFY50 | cI DBD fused to AbrB 48-88 | This study |
| pFY51 | cI DBD fused to SpoVT 4-54 | This study |
| pFY52 | cI DBD fused to SpoVT 4-178 | This study |
| pFY53 | cI DBD fused to AbrB 4-53 E35G | This study |
| pFY54 | cI DBD fused to AbrB 4-53 R23S | This study |
| pFY55 | cI DBD fused to AbrB 48-94 N64I | This study |
| pFY56 | cI DBD fused to AbrB 4-94 R23S | This study |
| pFY58 | cI DBD fused to AbrB 48-94 C54Y | This study |
| pFY59 | cI DBD fused to AbrB 48-94 C54S | This study |
| pFY60 | cI DBD fused to AbrB 48-94 C54W | This study |
| pFY61 | cI DBD fused to AbrB 4-53 I43S | This study |
| pFY64 | cI DBD fused to AbrB 48-94 C54Y | This study |
| pDR67 | pSpac, amyE integrative vector | |
| pFY66 | pSpac-(abrB 1-50) | This study |
| pFY80 | pSpac-(abrB 1-50)-gly-(spoVT 55-178) | This study |
| pFY81 | pSpac-(abrB 1-50)-gly-(abh 54-92) | This study |
| Phage-S | ||
| λKH54 | λΔcI mutant | J. Hu |
| λimm21C | Heteroimmune control | J. Hu |
| λ supervir | Supervirulent mutant of λ | D. Friedman |
| λKH54h80 | λΔcI h80 (i.e. host range of φ80) | J. Hu |