Table 2.

HCCorg-2T hepatocellular carcinoma organoid growth inhibition 50 (GI₅₀) values by different assay formats.

HCCorg-2T organoid Growth Inhibition Assay Format
Compound	GI50 μM			EC50 μM
TKI	CTB	HS-Orgs	CAM	EtHD
Sunitinib	65.2	29.5	19.5	NC
Crizotinib	43.7	80.6	<9	NC
Nexavar	64.6	>300	37.1	17.7
Imatinib	>300	>300	79.0	66.2

GI₅₀ is the concentration of compound that gives a 50 % growth inhibition response halfway between 0 % and 100 %. EC₅₀ is the concentration of compound that gives a 50 % increase in dead cell staining response halfway between 0 % and 100 %. TKI = tyrosine kinase inhibitor. CTB = cell titer blue viability reagent. HS-Orgs = number of Hoechst-stained organoids quantified by the multi-wavelength cell scoring (MWCS) image analysis module. CAM = the mean integrated intensity of the Calcein AM live cell staining quantified by the MWCS image analysis module. EtHD = the mean integrated intensity of the ethidium homodimer dead cell staining quantified by the MWCS image analysis module. NC = not calculable.