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Cellular levels of MutS. The amount of MutS, as determined by Western blotting, is shown for (lane a) AB1157, wild-type control; (lane b) GM4799 (mutS458)/pMQ372 (mutS+); (lane c) GM8311 (mutS+-bla); (lane d) GM8313 (mutSΔ2-bla); (lane e) GM8315 (mutSΔ800-bla); and (lane f) GM4799 (mutS458)/pmutSΔ800. Equal amounts of cell extract were loaded in each lane. There was no detectable MutS in extracts of strain GM4799 (data not shown).
