Figure 10.

Cardioprotective stimuli induce association of activated Akt with zyxin. Lysates (300 mg total protein) prepared from cultured cardiomyocytes treated with vehicle (control) or ANP (10^–9 M for 30 or 60 minutes) were immunoprecipitated with anti–phospho-Akt⁴⁷³ antibody, and presence of zyxin was detected by subsequent immunoblotting with anti-zyxin antibody. Whole cell lysate prior to immunoprecipitation is shown to indicate presence of zyxin (positive control). Immunolabeling with phospho-Akt⁴⁷³ antibody confirmed immunoprecipitation of protein (p-Akt). Immunoprecipitation with irrelevant antibody to anti-p16^ink4a is shown as a negative control. WB, Western blot.