Figure 12.

PKA inhibitor infusion into the CeA during ethanol intake and NPY levels in the amygdala of NP rats. (A) Low-magnification views of NPY mRNA expression (in situ RT-PCR; upper panels) and NPY gold immunolabeling (lower panels) in the CeA of NP rats with or without PKA inhibitor infusion during ethanol exposure. Inset shows the immuno-gold particles in a cell body at high magnification (×100). Scale bar: 40 μm. (B) Effects of voluntary ethanol exposure and PKA inhibitor infusion into the CeA on mRNA and protein levels of NPY in the CeA, MeA, and BLA structures of NP rats. Values represent the mean ± SEM of 5 rats in each group. *P <0.001 versus respective controls.