Table 2.
Sizes of DNA fragments produced by digestion of Buchnera genomes with selected restriction enzymes
| Buchnera spp. | Size of DNA fragments produced by digestion with restriction enzyme(s), kb
|
Total estimated DNA length, kb
|
||||
|---|---|---|---|---|---|---|
| KspI | ApaI | RsrII | ApaI + RsrII | Sum of restriction fragments, mean ± SD | Undigested gel | |
| APS* | 252, 176, 79, 52, 48, 34 | 286, 226, 73, 52, 3.4 | 277, 264, 99 | 240, 104, 99, 73, 52, 45, 24, 3.4 | ||
| APS | 255, 176, 85, 60, 48, 40 | 290, 235, 75, 52, 3.6 | 280, 280, 100 | 240, 108, 108, 75, 52, 45, 23, 3.6 | 659 ± 4 | 670 |
| MRO | 275, 170, 110, 110 | 475, 150, 46 | 420, 185, 65 | 669 ± 3 | 670 | |
| BPI | 560, 62, 30 | 550, 64, 30 | 640 | 550, 64, 30 | 645 ± 5 | 640 |
| TCA | 335, 225 | 565 | 570 | 565 | 565 ± 4 | 565 |
| THS | 315, 215 | 545 | 545 | 320, 234 | 544 ± 10 | 550 |
| CHP | 490 | 495, 25 | 515 | 435, 48, 25 | 508 ± 13 | 520 |
| CCE | 195, 115, 70, 70 | 440 | 450 | 405, 46 | 448 ± 5 | 450 |
| CCU | 185, 120, 70, 50, 24, 24 | 265, 135, 50, 25 | 475 | 200, 136, 64, 50, 30 | 476 ± 3 | 475 |
| CTU | 185, 120, 70, 50, 24, 24 | 265, 135, 50, 25 | 480 | 200, 136, 64, 50, 30 | 477 ± 4 | 475 |
*
Results deduced from the sequenced Buchnera sp. APS genome (4).