Table 2.
Analysis of TBP–DNA complexes with various transcription factors
| TBP mutant | Complex formationa | |||||
|---|---|---|---|---|---|---|
| TBP–DNA | BTAF1–TBP–DNA | BTAF1–TBP–mDNAb | TFIIA–TBP–DNA | NC2–TBP–DNA | TFIIB–TBP–DNA | |
| wild-type | + | + | + | + | + | + |
| V162A | − | + | + | + | ± | − |
| L185A | − | + | − | ± | − | ± |
| R186E | + | ± | − | + | ± | − |
| R188E | + | − | − | + | − | + |
| N189E | ± | − | n.d. | − | ± | + |
| E191K | − | + | + | + | + | − |
| K195A | + | + | n.d. | + | + | + |
| R208E | − | + | n.d. | ± | + | − |
| T210K | − | + | + | − | + | − |
| V240A | ± | + | + | + | ± | ± |
| Q242A | + | + | + | + | ± | ± |
| K243E | + | ± | n.d. | − | + | + |
| K249A | − | ± | − | − | − | − |
| K254A | − | + | + | + | + | − |
| K265A | − | + | + | + | + | ± |
| F288K | − | ± | − | − | − | − |
| R299A | − | + | + | + | + | ± |
| R318A | − | + | + | + | + | − |
aAnalysed in EMSA as described in Materials and Methods; plus, >50% of the wild-type TBP–DNA complex with indicated factor; plus-minus, 25–50% of the wild-type TBP–DNA complex with indicated factor; minus, <25% of the wild-type TBP–DNA complex with indicated factor.
bmDNA—EMSA probe bearing the mutated TATA-box (CGCAAACG).
n.d., not determined.