Figure 2.
Expression of E2F8. Western blot analysis of HeLa cells transfected with different E2F8 expression constructs. HeLa cells were transiently transfected with expression vectors encoding untagged or N-terminally tagged versions of hE2F8 or mE2F8 as indicated. pCMV mediates expression of untagged hE2F8 or mE2F8, while pCMV-Ha, pCMV-Myc, pGal and pVP16 express E2F8 fused to a Ha-epitope tag, myc-epitope tag, yeast Gal4 DNA binding domain and herpes virus simplex VP16 transcriptional activation domain, respectively. Expression of E2F8 was detected using a rabbit polyclonal antibody against murine E2F8. The positions of a standard set of molecular weight markers are shown on the left.