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. 2005 Sep 22;33(17):5458–5470. doi: 10.1093/nar/gki855

Figure 2.

Figure 2

Expression of E2F8. Western blot analysis of HeLa cells transfected with different E2F8 expression constructs. HeLa cells were transiently transfected with expression vectors encoding untagged or N-terminally tagged versions of hE2F8 or mE2F8 as indicated. pCMV mediates expression of untagged hE2F8 or mE2F8, while pCMV-Ha, pCMV-Myc, pGal and pVP16 express E2F8 fused to a Ha-epitope tag, myc-epitope tag, yeast Gal4 DNA binding domain and herpes virus simplex VP16 transcriptional activation domain, respectively. Expression of E2F8 was detected using a rabbit polyclonal antibody against murine E2F8. The positions of a standard set of molecular weight markers are shown on the left.

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