Table 1.

Dissociation constants (K_D, µM) for tryptophan

Isolate	L-Tryptophan Affinity chromatography	Ultrafiltration	Equilibrium dialysis	D-Tryptophan Affinity chromatography
Sequences containing the CYA motif
Trp 70–93	8/11			1100/1000
Trp 70–305	11/8	7
Trp 70–317	15			1200/1600
Trp 70–730	17/15	24		1200/1300
Trp 70–727	16/10	15	9	3600/3600
				2400/4800
Trp 70–151	15
Trp 70–91	15/7
Trp 70–92	9/8	13		2700/900
Trp 70–372	27/15	16		2000/4000
Trp 70–148	5/9
Sequences that do not contain the CYA motif
Trp 70–358	4/8
Trp 70–189	12/6
Trp 70–585	25/24

For affinity chromatography, independent measurements of K_D are presented. See Materials and Methods for K_D calculations.