Table 2.
Summary of selections
Initially randomized length | 70 | 60 | 40 | 20 | 17 |
Final pool at selection cycle | 8 | 4 | 4 | 5 | 9 |
RNA recovery in final elution | 18/17%a | 26% | 34% | 17% | 5% |
Number of clones sequenced | 158 | 48 | 50 | 49 | 26 |
Abundance of the CYA motif | 81% | 67% | 72% | 63% | 0% |
Independent isolations, CYA motif | 19 | 11 | 15–16b | 4–6b | 0 |
aThe two figures correspond to two parallel selections as described in Materials and Methods.
bSee text for explanation of range of possible independent isolations.