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Mitochondrial DNA is absent in mutants of the accessory subunit of Drosophila Pol γ. Three micrograms of total DNA that was isolated from third instar larvae was digested with XhoI, which cleaves mtDNA once; duplicate samples of the DNAs (1 μg) were electrophoresed in a 0.8% agarose gel, transferred to a nylon membrane, and hybridized with 32P-labeled DNA probes from the ATPase 6 gene of mtDNA and a repeated histone gene cluster as a nuclear DNA control.
