Figure 5.

PCR genotyping of founder and offspring genomic DNA. Offspring of an agouti founder were genotyped by using primers based on flanking sequences cloned from insertion site E (a) or insertion site B (b). In both cases, a second reaction was performed by using a transposon-specific primer and a primer in the flanking genomic DNA to detect the transposon insertion. (c) Mice heterozygous for the E insertion were intercrossed. Offspring were genotyped by PCR, and an example of a mouse with each genotype is shown.