Table 1.

Summary of Southern blot analysis

mRNA injected	No. of embryos		Transgenic frequency	Type of insertion		Avg. SB-mediated copy number
	Total	Transgenic		SB-mediated	Random
SB	42	19	45%	6	13	3
GFP	21	6	29%	0	6	NA

Data from 4 days of pronuclear coinjection are shown. The data in the left portion of the table shows the total number of embryos analyzed for each type of mRNA injected along with the number of transgenic embryos determined by Southern blotting. The right portion of the table summarizes the Southern blot analysis. Transgenic embryos were classified as having SB-mediated insertions if they had a band on Southern blot that hybridized to the GFP probe but not the plasmid probe. Also shown is the average transposon copy number for each of the 6 transgenic embryos containing SB-mediated insertions.