Fig. 2.

The occurrence of variable splicing events with significant differences suggested the regulatory role of METTL3_14 in PANC-1 cell line. (A) The bar plot showing the number of all significant regulated alternative splicing events (RASEs). X-axis: RASE number. Y-axis: the different types of AS events. (B) Principal component analysis (PCA) of siMETTL3_14 and siC samples in PANC-1 cell line based on FPKM value of all significant NIR RASEs based on PSI. AS filtered should have detectable splice junctions in all samples and at least 80% samples should have > = 10 splice junction supporting reads. The ellipse for each group is the confidence ellipse. (C) Hierarchical clustering heatmap of all significant NIR RASEs based on PSI. AS filtered should have detectable splice junctions in all samples and at least 80% samples should have > = 10 splice junction supporting reads. (D) Bar plot exhibited the most enriched GO biological process results of the NIR regulated alternative splicing genes (RASGs). (E) Venn diagram showing the overlap genes number of NIR RASGs and DEGs