Figure 4.

A combined absence of NF-κB1 and c-Rel prevents mitogen-induced B cell growth. Normal, c-rel^−/−, nfkb1^−/−, and nfkb1^−/−c-rel^−/− splenocytes stimulated in culture with anti-RP antibodies plus LPS for 72 h were stained at 24-h intervals with biotinylated B220, then examined by flow cytometry. Forward light scatter profiles (x axis: linear scale) are a measure of cell size. The data shown were electronically gated to exclude dead cells and are representative of three experiments. Similar results were obtained by using different stimuli (results not shown).