Figure 1.

Peptide–MHC affinity of the MHC variant ligands. (A) Purified IE^k and biotinylated reference peptide, MCC 88–103, were incubated at 37°C for 48 h. This reaction mixture and the indicated concentrations of competitor peptides were added to a 96-well plate, coated overnight with an IE^k capture antibody. (B) Relative IC₅₀ is the amount of peptide needed to inhibit the binding of a high affinity biotinylated reference peptide by 50%, normalized to a value of one for MCC.